منابع مشابه
Polygalacturonase of Erwinia carotovora.
The polygalacturonase, clearly differentiated from polygalacturonic acid trans-eliminase, produced in the culture fluid of Erwinia carotovora was purified 56-fold by means of acetone precipitation, acetate butfer extraction, and two successive treatments by chromatography on carboxymethyl cellulose. The purified enzyme is specific for nonmethoxylated polygalacturonic acid. It shows an optimum p...
متن کاملEvidence that polygalacturonase is a virulence determinant in Erwinia carotovora.
Polygalacturonase (PG) was purified from Erwinia carotovora EC. A hybrid cosmid, pSH711, that encodes PG activity but not pectate lyase activity was identified from an E. carotovora genomic library by an immunological screening method. A cell extract of Escherichia coli cells containing pSH711 was able to produce plant tissue maceration when spotted on carrot, potato, or turnip slices. In addit...
متن کاملL-Asparaginase from Erwinia carotovora
The physicochemical properties of the antileukemic Lasparaginase from Erwinia carotovora have been studied and compared with those of the corresponding clinically effective enzyme from Escherichia coli B. Although both enzymes are tetramers with similar molecular weight, the amino acid compositions are distinctly different and the Erwinia enzyme is more basic as judged by polyacrylamide electro...
متن کاملBiological Control of Erwinia carotovora ssp. carotovora by Streptomyces Species
Ten isolates of Erwinia carotovora ssp. carotovora (Ecc) were isolated from infected potato tubers of Picasso, Sante, and Nevskiy varieties collected from different regions in Kyrgyzstan. Isolates were identified as Erwinia carotovora ssp. carotovora (Ecc) by standard bacteriological techniques and pathogenicity tests on tubers and also by PCR analyses. Tests on the pathogenicity of E. carotovo...
متن کاملObservations on the bacteriophagy of Erwinia carotovora.
The lysis of Erwinia carotovora by bacteriophage has been reported by Coons and Kotila (1925), but the morphological characteristics of the virus could not be studied at that time, prior to the methods of electron microscopy, and some of the important physiological characteristics have not been investigated. In undertaking to obtain further data on these aspects of the problem, we have obtained...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1966
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)96430-6